Effects of miR-133a precursor and miR-133 inhibitor on human islet insulin biosynthesis.

<p>Human islets were transfected with miR-133a precursor or inhibitor for two days and then cultured for 24 hours at 5.6 or 20 mM glucose. Insulin and total protein biosynthesis was quantified as given above using 10–20 islet equivalents per group. Results are means ± SEM for 3–4 observations.</p><p>*denotes p<0.05 using Student's t-test when comparing vs. control. The percentages insulin biosynthesis per total protein biosynthesis in these experiments are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010843#pone-0010843-g005" target="_blank">Figure 5B</a>.