Bisphenol A (BPA) content and vitellogenin (VTG) expression in trout embryos.

<p>Temporal changes in BPA concentrations (A) and VTG mRNA abundance (B) and protein expression (C) in rainbow trout embryos after 3 h acute exposure of oocytes to low (30 µg. ml⁻¹) and high (100 µg.ml⁻¹) dose of BPA. BPA was dissolved in ethanol and added to the ovarian fluid and after 3 h exposure oocytes were fertilized with untreated sperm, water hardened and maintained at 8.5°C. BPA was quantified in embryos collected soon after exposure (3 h) and at 13-, 21- and 35-dpf using LC-MS/MS method. The minimum detection limit of BPA was 75–80 ng.g⁻¹ wet weight of tissue (n = 3; each replicate is a pool of 6 embryos). nd denotes non-detectable levels of BPA. VTG mRNA levels in embryos at 13- and 140-dpf (B) were determined by quantitative real-time PCR (qPCR). Liver VTG protein expression (C) was determined in the juveniles at 140-dpf by Western blotting. VTG protein content was detected using anti-trout VTG antibody raised in rabbit. VTG protein was non-detectable (nd) in the control group; values represent mean + SEM (n = 6–8 fish); bars with different letters are statistically significant (ANOVA, p<0.05).