Effect of CD133 on cell motility.

<p>Transwell migration was measured after knockdown of CD133 in HT29 and sorting of SW480 into CD133+/− populations. Significantly fewer HT29^CD133− cells migrated across the membrane than HT29^ssc cells (Figure 6a, p = 0.04). Conversely, larger numbers of sorted CD133+ cells migrated than CD133− cells (Figure 6b, p = 0.03). A wounding assay was also undertaken and gene knockdown was associated with marked delay in closure of the wound which was visiually perceptible after only 24 hours (Figure 6c) and statistically significant (p<0.001).