The recipient inflammatory response after UMSCs and UHSCs transplantation into <i>Lum^-/-</i> cornea.

<p><b>A.</b> Immunostaining with antibodies against inflammatory cells, e.g., CD45 (leukocyte), CD90 (T-Cell) and F4/80 (macrophage) demonstrated the presence of large numbers of immune and inflammatory cells in UHSC transplanted corneas but not in UMSC transplanted corneas, indicating that UMSCs might suppress the host immune response. <b>B.</b> More CD45+ leukocytes invaded UHSC-recipient corneal stroma after the transplantation and the peak of leukocyte infiltration occurred one week after transplantation (n≥5 in each time point). <b>C.</b> The recipient F4/80+ macrophages were present in corneas transplanted with UMSCs and UHSCs. In contrast, more macrophages were found in corneas with UHSC transplantation than that of UMSC transplantation (n≥5 in each time point). <b>D.</b> The infiltration of recipient CD90+ T-cells appeared at the first week and maintained at high levels after UHSC transplantation; very few CD90+ cells were seen after UMSCs transplantation (n≥5 in each time point). Scale bars: 10 µm; Blue: DAPI.