Distamycin A selectively decreases induction of P-selectin promoter activity and expression.

<p><b>A.</b> BAEC cells were transiently transfected with promoter-reporter constructs for P-selectin and E-selectin. Transfected cells were treated with Vehicle, TNF-α/Vehicle, or TNF-α/Dist A, then harvested twelve hours after treatment and assessed for luciferase activity (with normalization for β-galactosidase levels). Similar experiments were performed in which transfected cells were treated with Vehicle, LPS/Vehicle, or LPS/Dist A and harvested four hours after treatment. Fold change was assessed relative to normalized values of “1” for the Vehicle-treated condition for each construct. These experiments were repeated 3 separate times with duplicate wells for each condition. (*p<0.05 compared with Vehicle for each construct; **p<0.05 compared with TNF-α/Vehicle or LPS/Vehicle for P-selectin). <b>B.</b> Lung tissue was harvested from wild type mice two hours following treatment with Vehicle (Veh), LPS/Vehicle, or LPS/Dist A (Dist), then subjected to RNA extraction and Northern blotting using a radiolabeled probe for P-selectin or E-selectin (and an 18S probe as loading control). This experiment was repeated two separate times.