A melanoma-reactive serum IgG response was found in MIC-treated mice.

<p>Upon sacrifice peripheral blood serum was obtained from treated mice, and serum antibody binding to B16.F10 melanoma cells was analyzed using flow cytometry (n = 5 mice per group). EL4 syngeneic thymoma cells were used as a control to verify the melanoma-specificity of the antibody binding, and showed comparable serum IgG binding levels in all groups (p>0.05, one-way ANOVA). A significant level of IgG antibody binding to the melanoma cells above the EL4 background level was found in the MIC-treated mice (p<0.01, paired t-test). Furthermore, only the MIC-treated mice showed melanoma-reactive IgG levels significantly above those found in untreated mice (p<0.02, unpaired t-test). IgA controls were negative, and IgM antibodies showed no significant binding levels (data not shown). Data is representative of three independent <i>in vivo</i> experiments.