Syn-GFP is present in neuronal cell bodies and presynaptic terminals.

<p>A. Fixed tissue confocal image of Syn-GFP in the cortex shows one neuronal soma and that the vast majority of neuropil puncta staining is present within axons. Endogenous GFP fluorescence is shown without antibody labeling. B. <i>In vivo</i> multiphoton image of Syn-GFP in the cortex shows staining in one neuronal soma and multiple neuropil puncta. Scale bar for A–B 10 µm. C. Fixed tissue confocal image from the cortex of a Syn-GFP animal showing GFP staining in two somata and in the neuropil. D. Staining in the same section for human α-synuclein. E. Merged image shows colocalization between GFP signal and human α-synuclein, as expected for this fusion protein. Scale bar for C–E 20 µm.