Down-regulation of Cux1 by RNAi increases the dendritic complexity of cortical pyramidal neurons.

<p>(A–B) RT-PCR analysis of Cux expression level following RNAi treatments. Two specific RNAi each designed to different regions of ORF for Cux1 and Cux2, Cux1/2-RNAiA and -RNAiB, could result in the down-regulation of their expression levels. Representative images are semi-quantitative PCR. Quantitative results are measured by real-time PCR. Data shown are mean ± SEM (n = 3 independent experiments). * <i>P</i><0.05, ** <i>P</i><0.01, paired <i>t</i> test. (C–E) Increase of pyramidal neuron dendritic complexity after transfection of Cux1-RNAi plasmids. Representative cells are shown in (C). Scale bars: 50 µm. Data shown are mean ± SEM (n≥30 for each condition). ** <i>P</i><0.01, Student's <i>t</i> test. (F–G) Dendritic morphology of pyramidal neurons after transfection of Cux2-RNAi plasmids. Data shown are mean ± SEM (n≥30 for each condition). <i>P</i>>0.05, Student's <i>t</i> test.