Zebrafish TS protein interacted with its own mRNA in vivo.

<p><i>A</i>, Western blot analysis was performed with purified recombinant zebrafish TS (lane 1) or protein from the immunoprecipitation complex (lane 2) and detected with human TS106 antibody. <i>B</i>, RT-PCR products using the RNA extracted from immunoprecipitation complex as the template. TS mRNA from nt 340–1040 was amplified by RT-PCR using TS-specific primers (lanes 4 and 5). RNA and protein complex was precipitated without TS antibody and RT-PCR was performed (lane 2). Lane 3 indicates the RT-PCR results precipitating the complex with unrelated polyclonal antibody, CHX10-like zebrafish antibody (GenWay Biotech, CA). Lane 1 represents the DNA marker of molecular weight.