Construction of a <i>B. melitensis</i> Protein Microarray.

<p>Arrays were printed containing 1406 <i>B. melitensis</i> proteins, positive and negative control spots. Proteins were printed in duplicates. Each array contains positive control spots printed from 6 serial dilutions of human and mouse IgG, 6 serial dilutions of EBNA1 protein, and “No DNA” negative control spots. (A) The array was probed with anti-His antibody as described in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0000673#s2" target="_blank">Materials and Methods</a>, to confirm the expression and printing of over 95% proteins. (B) Comparison of arrays probed with Peruvian naïve serum and Culture positive serum. The arrays were read in a laser confocal scanner, analyzed, and the data normalized as described in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0000673#s2" target="_blank">Materials and Methods</a>. The signal intensity of each antigen is represented by rainbow palette of blue, green, red and white by increasing signal intensity.