Changes in SmAQP expression.

<p>A. Relative SmAQP expression (mean ± SE) in adult schistosomes 7 days after treatment with none, or control, or SmAQP siRNA. B, Detection by western analysis of SmAQP protein (top panel), and a control protein (SPRM1hc, bottom panel) in extracts prepared from parasites 28 days after treatment with none (lane 1), control (lane 2), or SmAQP (lane 3) siRNA. The arrowhead indicates the diminished level of SmAQP protein seen in lane 3. C. Localization of SmAQP in whole, fixed adult parasites stained with anti-SmAQP antiserum (a–h) 14 days after no (a,b), control (c,d), or SmAQP (e,f) siRNA treatment. An additional control group (g,h) was untreated with siRNA and stained with secondary antibody alone. Panels a,c,e,g are phase contrast images while panels b,d,f,h illustrate antibody binding. Non suppressed parasites (panels a–d) stain brightly for SmAQP while the SmAQP-suppressed parasites (e,f) and parasites stained with secondary antibody alone (g,h) stain very weakly. The bar represents 100 µm.