<p>(<b>A</b>) Immunoblots using 50 µg protein of total cell lysate and subcellular fractions (mitochondria and nuclear fraction obtained from a sucrose gradient) were subject to SDS-PAGE and subsequently transferred to PVDF filters (Immobilon, Millipore Corp.). Blots were then blocked with BSA and initially probed using a mouse monoclonal antibody specific for p53 (clone 1801, Oncogene Research) followed by HRP-conjugated anti-mouse IgG (Roche) and then developed using ECL kit (GE Healthcare/Amersham). Blots were reprobed using mouse monoclonal specific for the mitochondrial marker, Cox II (Molecular Probes, Invitrogen) and nuclear marker PCNA (Calbiochem) followed by HRP-conjugated anti-mouse IgG and then developed using the ECL kit (GE He...
<p>(A) Empty vector- and p53-transduced G55T2 populations were monitored by FACS, 4 and 8 days post ...
AIMS: To establish whether PAb248 recognises human p53 as well as murine p53 and if so, to determine...
<p><b>(A)</b> Representative raw immunofluorescence images of fixed MCF7 (top row) and BE (bottom ro...
<p>(<b>A</b>) Various deletions of FLAG –tagged p53 were used to determine involvement in mitochrond...
<p><b>(A)</b> Schematic of the p53-associated phenotypes. <b>(B)</b> Cell viability, senescence-asso...
<p>Immunocytochemical assay was performed using biotin-streptavidin-HRP method with monoclonal mouse...
The tumour suppressor p53 is considered to be one of the most studied proteins of all time, and yet ...
<p>(A) Western blot analysis of <i>Rnf8<sup>−/−</sup>p53<sup>−/−</sup></i> and control MEFs for p21 ...
<p>Panel ‘A’ photomicrographs shows liver section of control mice (group 1), Immunostained for p53 w...
<p>(A) MNNG induced the nuclear translocation of p53 protein. The localization of the p53 protein wa...
<p>(A) Quantitative RT-PCR analysis was performed for each sample in triplicate. The median ΔΔC<sub>...
<p>A) Cycloheximide chase in MCF-7 and MCF-7Skp2B cells was performed for the indicated time and cel...
<p>D54 cells with an ectopic expression <i>(A)</i> or shRNA depletion <i>(B)</i> were mock treated o...
<p>(<b>A</b>) MSC derived from p53 knockout mice (MSC-p53KO) were exposed (IR) or not (CTRL) to 10 G...
<p>(A) Accumulation of p53 was investigated by Western Blot using p53 antibody in lysate from cells ...
<p>(A) Empty vector- and p53-transduced G55T2 populations were monitored by FACS, 4 and 8 days post ...
AIMS: To establish whether PAb248 recognises human p53 as well as murine p53 and if so, to determine...
<p><b>(A)</b> Representative raw immunofluorescence images of fixed MCF7 (top row) and BE (bottom ro...
<p>(<b>A</b>) Various deletions of FLAG –tagged p53 were used to determine involvement in mitochrond...
<p><b>(A)</b> Schematic of the p53-associated phenotypes. <b>(B)</b> Cell viability, senescence-asso...
<p>Immunocytochemical assay was performed using biotin-streptavidin-HRP method with monoclonal mouse...
The tumour suppressor p53 is considered to be one of the most studied proteins of all time, and yet ...
<p>(A) Western blot analysis of <i>Rnf8<sup>−/−</sup>p53<sup>−/−</sup></i> and control MEFs for p21 ...
<p>Panel ‘A’ photomicrographs shows liver section of control mice (group 1), Immunostained for p53 w...
<p>(A) MNNG induced the nuclear translocation of p53 protein. The localization of the p53 protein wa...
<p>(A) Quantitative RT-PCR analysis was performed for each sample in triplicate. The median ΔΔC<sub>...
<p>A) Cycloheximide chase in MCF-7 and MCF-7Skp2B cells was performed for the indicated time and cel...
<p>D54 cells with an ectopic expression <i>(A)</i> or shRNA depletion <i>(B)</i> were mock treated o...
<p>(<b>A</b>) MSC derived from p53 knockout mice (MSC-p53KO) were exposed (IR) or not (CTRL) to 10 G...
<p>(A) Accumulation of p53 was investigated by Western Blot using p53 antibody in lysate from cells ...
<p>(A) Empty vector- and p53-transduced G55T2 populations were monitored by FACS, 4 and 8 days post ...
AIMS: To establish whether PAb248 recognises human p53 as well as murine p53 and if so, to determine...
<p><b>(A)</b> Representative raw immunofluorescence images of fixed MCF7 (top row) and BE (bottom ro...