Effect of manganese on polymerase activity and misincorporation efficiency of IAV Pol complex.

<p>(<b>A</b>) IAV polymerization with 5 mM Mg(OAC)₂ and varying concentration of MnCl₂ (lanes 1 to 4, 0.5, 1, 5, 7.5 mM). LC: loading control (<b>B</b>) Fully extended products for the IAV Pol polymerase activity with different metal concentrations were compared after being normalized with the value obtained with 5 mM Mg(OAC)₂ (100%). (<b>C</b>) The misincorporation efficiency of IAV Pol at 0.5 mM MnCl₂ was calculated as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010372#pone-0010372-g002" target="_blank">Figure 2F</a>, and compared with the value obtained with 5 mM Mg(OAC)₂ (100%). C: no polymerase control.