The LBH589 and TRAIL combination augments caspase activation (<i>A</i>) and apoptosis (<i>B</i>).

<p><i>A</i>, The indicated cell lines were treated with DMSO control, 50 nM LBH589 25 ng/ml TRAIL alone, or LBH589 plus TRAIL. After 16 h, the cells were subjected to preparation of whole-cell protein lysates for detecting caspase cleavage using Western blotting. Casp, caspase; CF, cleaved fragment. <i>B</i>, Panc-1 cells were treated with 25 ng/ml TRAIL alone, 50 nM LBH589 alone or their combination for 24 h. The cells were then subjected to measurement of apoptosis using annexin V staining. The percent positive cells in the upper right and lower right quadrants represent the total apoptotic cell population.