Enforced expression of ectopic c-FLIP, but not survivin, confers cell resistance to caspase activation (A) and apoptosis (B) induced by the LBH589 and TRAIL combination.

<p>The indicated transfectants were treated without and with 50 nM LBH589 plus 25 ng/ml TRAIL (L/T) for 16 h (<i>A</i>) or 24 h (<i>B</i>). The cells were then harvested for preparation of whole-cell protein lysates for detecting caspase and PARP cleavage using Western blotting (<i>A</i>) or for measurement of apoptosis using annexin V staining (<i>B</i>). The percent positive cells in the upper right and lower right quadrants represent the total apoptotic cell population.