Vascular leakage in D2Y98P-infected mice.

<p>AG129 mice were inoculated ip. with 10⁷ or 10⁴ PFU of D2Y98P. At day 6 p.i. (10⁴ PFU dose) or at moribund state (both doses), mice were intravenously administered with Evans blue. After 2 hours, they were perfused extensively with PBS and assessed for Evans Blue extravasation in tissues. (A) Evan's blue extravasation in the peritoneal cavity (top panel) and intestines (bottom panel) of uninfected or D2Y98P-infected mouse at moribund state. (B) Quantification of Evans blue dye in the intestine, liver and spleen from mice. Five animals per group per time point were individually processed. Data are expressed as the [mean ± SD] of fold increases in OD_620nm per gram of wet tissue compared to uninfected controls. (C) Serum albumin concentration. Results are expressed as the [mean ± SD] of 5 animals per time point per group. *p<0.05. Results are representative of 2 independent experiments.