Quantitative analysis of <i>53R</i> mRNA levels in cells co-transfected with different plasmids.
- Yu-Sin Kim (365341)
- Fei Ke (365342)
- Xiao-Ying Lei (311837)
- Rong Zhu (172093)
- Qi-Ya Zhang (311840)
Publication date
February 2013
DOIAbstract
<p>GCO cells co-transfected with pSM155-amiR-53R-1/pEGFP-N3-53R, pSM155-amiR-53R-2/pEGFP-N3-53R and pSM155-amiR- 53R-3/pEGFP-N3-53R, respectively. <i>53R</i> mRNA level from each group was measured by real-time PCR analysis 72 h post transfection. Group co-transfected with pSM155-amiR-PB2/pEGFP-N3-53R was used as negative control. The value of negative control was designated as 1.0 (n = 3). The values represent averages of three independent experiments, with the range indicated (±S.D). *P<0.05 versus control.</p
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