Chaperone-like effect of anti-ACE mAbs on mutant ACE.

<p>Membrane-bound (<b>A</b>) and soluble (<b>B</b>) mutant ACE (Q1069R) were pre-incubated with N-domain specific mAb 9B9 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010438#pone.0010438-Danilov4" target="_blank">[30]</a> or C-domain specific mAbs 1B3 and 1B8 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010438#pone.0010438-Naperova1" target="_blank">[16]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010438#pone.0010438-Balyasnikova6" target="_blank">[36]</a> at the indicated concentrations for 2 hrs at room temperature and then residual activity was determined with 5 mM HHL and 2 mM ZPHL as substrates. Experimental conditions are as described in the legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010438#pone-0010438-g005" target="_blank">Fig. 5</a>. Data presented as ZPHL/HHL ratio of ACE activity in the presence mAb. Results shown are the mean ± SD of 3–4 experiments.