Isolation and characterization of single nucleotide polymorphisms in the common carp (Cyprinus carpio L.) genome

In this study, we constructed a genomic library to identify a set of new SNPs from the common carp (Cyprinus carpio L.) genome. Putative SNPs were validated by amplification of 41 fragments from the library using unrelated individuals. A total of 211 SNPs, 18 Indels and 6 microsatellites were identified from a total length of 18,244 bp genomic sequences. A/G was the most common substitution, and the ratio of transitions to transversions was 2.3:1. Ten validated SNPs were selected for genetic diversity analysis in three wild populations of common carp, and all loci were polymorphic, with the average observed (H (o)) and expected (H (e)) heterozygosities were 0.2154 and 0.3321, respectively. These new SNPs would be good resources for population genetics, parentage analysis and conservation genetics in common carp.In this study, we constructed a genomic library to identify a set of new SNPs from the common carp (Cyprinus carpio L.) genome. Putative SNPs were validated by amplification of 41 fragments from the library using unrelated individuals. A total of 211 SNPs, 18 Indels and 6 microsatellites were identified from a total length of 18,244 bp genomic sequences. A/G was the most common substitution, and the ratio of transitions to transversions was 2.3:1. Ten validated SNPs were selected for genetic diversity analysis in three wild populations of common carp, and all loci were polymorphic, with the average observed (H (o)) and expected (H (e)) heterozygosities were 0.2154 and 0.3321, respectively. These new SNPs would be good resources for population genetics, parentage analysis and conservation genetics in common carp