Naujas galvos smegenų išemijos modelis neuroprotektorių tyrimams

Cerebral ischemia is one of the foremost causes of high mortality and morbidity for both developed and developing countries. Cerebral ischemia occurs commonly in patients who have a variety of clinical conditions including cardiac arrest (CA), shock, and asphyxia and in patients undergoing complex cardiac surgery. Stroke is an important cause of morbidity and mortality in industrialized countries and few therapies exist thus far. Despite recent advances in out-of-hospital cardiac arrest resuscitation, hypoxic-ischemic brain damage still causes considerable mortality and morbidity. A better understanding of clinical and cell physiological alteration is crucial to develop therapeutic strategies. Stroke and cerebral ischemia continues to kill millions people each year, and the development of safe and effective treatment is a major challenge to experimental and clinical neuroscience. The aim of the study was to create a new experimental swine model of global incomplete cerebral ischemia and to check neuroprotective effect of methylene blue and cyclosporine A in created model. Tasks of the study: 1) to evaluate mitochondrial respiration, histological and microcirculatory changes in experimental models of global cerebral ischemia, 2) to evaluate mechanisms and identify the primary target of ischemia in brain cell in experimental models, 3) to evaluate neuroprotective effect of methylene blue and cyclosporin A in created swine model of global cerebral ischemia