Stafilokokų išskyrimas iš gyvūnų augintinių klinikinės medžiagos ir jautrumo antimikrobinėms medžiagoms nustatymas

The aim of this work was to investigate the occurrence and antibiotic susceptibility of Staphylococcus species isolated from clinical material collected from pets, and the implications of these findings. A retrospective analysis of 78 Staphylococcus-positive samples from cats and dogs was made. The specimens were submitted between January 2016 and November 2017, and collected from the skin, outer ear, ocular conjunctiva and urogenital tract. 89.7% (70/78) were identified as S. pseudintermedius, 7.7% (6/78) as S. aureus, and 2.6% (2/78) as S. epidermidis. Resistance among isolates was highest to polymyxin B (91%) and sulfamethoxazole (82%). Resistance was lowest to enrofloxacin (14%) and gentamicin (16%). 77% of all staphylococcal isolates displayed resistance to at least one antimicrobial, and 30% were multi-drug resistant. Antimicrobial resistance and multi-drug resistance were more common in S. aureus than in S. pseudintermedius. A separate study involving clinical material from 28 Staphylococcus-positive dogs collected between April 2017 and October 2017 was made, where species identification, antimicrobial susceptibility against five common beta-lactams and beta-lactamase production were investigated using standard biochemical diagnostic methods and disc diffusion test. Specimens were collected from skin, outer ear, and the urogenital tract. All isolates were identified as S. pseudintermedius. Antimicrobial resistance was highest to ampicillin (96%), and penicillin G (93%). Sensitivity was highest to amoxicillin + clavulanic acid (79%). Beta-lactamase production was noted in 90% (10/11) of the isolates tested. The staphylococci were most frequently isolated from the skin (61%) in canines and the ocular conjunctiva (43%) in felines. S. pseudintermedius (91%) and S. epidermidis (2.9%) were more common in canines, whereas S. pseudintermedius (80%) and S. aureus (20%) were more common in felines. There was no correlation between age or the gender of the animals, and the staphylococcal species isolated. No collection site was more associated with a certain staphylococcal species or resistance to a particular type of antimicrobial agent. This indicates that most common infection site varies between canines and felines, but primary factors regarding susceptibility to infection does not include either age, gender or bacterial species. Furthermore, antimicrobial resistance could not be linked to site of infection in this study