Additional file 5: of The role of fibrinolysis inhibition in engineered vascular networks derived from endothelial cells and adipose-derived stem cells
- Severin Mühleder (4847977)
- Karoline Pill (4847959)
- Mira Schaupper (4847965)
- Krystyna Labuda (4847950)
- Eleni Priglinger (4847953)
- Pablo Hofbauer (4847962)
- Verena Charwat (1876324)
- Uwe Marx (1941607)
- Heinz Redl (70157)
- Wolfgang Holnthoner (673125)
Publication date
February 2018
DOIAbstract
Thrombin transiently activates EC. HUVEC-ASC clots were fixed and immunofluorescence was performed against E-Selectin after 4 h, 1 day, 4 days and 7 days. Staining revealed transient activation of EC via thrombin after 4 h. TNF-α (10 ng/ml), serving as a positive control, activated EC more strongly after 4 h than thrombin alone. No aprotinin was used in any sample. n = 8 from two independent experiments. Scale bar: 200 μm. (DOC 322 kb
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