Additional file 3: of The role of fibrinolysis inhibition in engineered vascular networks derived from endothelial cells and adipose-derived stem cells

Influence of different aprotinin concentrations on HUVEC network formation. (A) Representative images of the effect of different aprotinin concentrations (0 KIU/ml, 5 KIU/ml, 10 KIU/ml, 20 KIU/ml, 30 KIU/ml and 100 KIU/ml) on HUVEC/ASC vascular network formation taken on day 28 of incubation. (B) Quantification of the network by number of junctions, tubules, total and mean tubule length. Increased aprotinin concentration results in a decreased number of tubules as well as junctions and total tubule length. Mean tubule length shows a dose-dependent increase, which peaks in samples with 20 KIU/ml aprotinin. Values are from two independent experiments using two different ASC donors; n = 2. Scale bar: 200 μm. (DOC 741 kb