Crosslinking of Tc DNA polymerase β to kinetoplastid DNA.

<p>(A) <i>T</i>. <i>cruzi</i> epimastigote and trypomastigote forms were treated with peroxide. Proteins were crosslinked to the DNA with formaldehyde and then DNA was sheared and immunoprecipitated with antibodies against Tc DNA polymerase β (αDNA β-Pol) or control IgG. Revert-crosslinked DNA precipitated (IP) was purified and amplified by PCR to amplify nuclear satellite DNA (S DNA) or kinetoplastid DNA (K DNA). (B) Quantification of PCRs amplifying S DNA and K DNA are indicated above the gel panel. Results are indicated as the mean +/- SD of three independent experiments. Input (IN) corresponded to 2% of total starting DNA and precipitated DNA (IP) represents 50%. * indicates <i>p</i> < 0.05.