Effects of SV40 infection on proliferation of human lymphocyte cell lines.

<p>Human B cells (A) DG75 and (B) BJAB and T cells (C) CEM were infected with SV40 strains 776-1E, 776-2E, and the miRNA mutant 776-2E-SM1. Cells were collected at indicated time points after infection and live cells were determined by trypan blue exclusion. After counting, cell densities were adjusted to 1 × 10⁶ cells/ml for the next incubation period. Changes in cell numbers between harvests are plotted. Both DG75 and BJAB cells infected by the 776-2E-SM1 miRNA-null mutant showed somewhat slowed growth compared to the mock-infected cells, but remained viable.