The forms of prostasin, matriptase, HAI-1 and HAI-2 in human foreskin.

<p>Human foreskin lysates were prepared from 4 different donors in two batches with specimen 1–3 together and specimen 4 alone. Equal amount of protein from the four tissue lysates were analyzed by immunoblot for prostasin, matriptase, and HAI-1, as indicated (A and B). HAI-2 species were also analyzed in specimens 1–3 (C). The prostasin species include prostasin zymogen, active prostasin, and prostasin complex with HAI-1, as indicated. Matriptase species include matriptase zymogen and matriptase complex with HAI-1, as indicated. HAI-1 species include HAI-1 monomer, HAI-1 complexes with prostasin or matriptase, as indicated. HAI-2 species include the one with heavy N-glycan (C, right panel) and the one with light N-glycan (C, left panel).