Ablation of <i>LmxM</i>.<i>22</i>.<i>0010</i> by CRISPR-Cas9.

<p>A, Schematic representation of the WT and recombined alleles after replacement with Puro resistant gene. Annealing positions of the probes and expected fragment sizes are shown. B, Southern blot analysis of the <i>Bst</i>E II digested <i>L</i>. <i>mexicana</i> genomic DNA of the WT, Cas9, and BTN1 ablated strains (labeled KO) with <i>LmxM</i>.<i>22</i>.<i>0010</i> 5' UTR, <i>LmxM</i>.<i>22</i>.<i>0010</i> 3' UTR and Puro probes.