Ablation of <i>LmxM</i>.<i>22</i>.<i>0010</i> by conventional approach.

<p>A, Schematic representation of the WT and recombined alleles after replacement with Sat-, Hyg-, Neo-, and Ble-resistant genes. Annealing positions of the probes and expected fragment sizes are shown. B, Southern blot analysis of the <i>Bst</i>E II digested <i>L</i>. <i>mexicana</i> genomic DNA of the WT and BTN1 ablated strains with Sat, Hyg, Neo, Ble, 5' UTR, and <i>LmxM</i>.<i>22</i>.<i>0010</i> ORF probes.