Inhibition of cFLIP_L expression sensitizes Huh 7 cells to anti-profiferative and pro-apoptotic effects.

<p>(A) Western blot was used to determine the cFLIP_L protein level after transfection with cFLIP_L siRNA for 24 h. (B-C) Transfected cells were treated with TRAIL in the presence or absence of shDcR3 for 24 h. IETDase colorimetric assay was used to determine the IETDase and DEVDase activities in cell lysates. (D) Western blot was used to detect the mitochondrial cytochrome c release. (E) CCK-8 assay was used to determine the cell viability. (F) Cells were transfected with mock or cFLIP_L vector for 24 h, cFLIP_L protein level was detected by western blot. In the presence or absence of shDcR3, transfected cells were treated with TRAIL for 4 h. CCK-8 was used to measure the cell viability. Each value represents the mean ± SEM of three independent experiments performed in triplicate. *P<0.05 and **P<0.01.