Effects of DcR3 knockdown on apoptosis-regulatory proteins.

<p>In the presence or absence of shDcR3 and z-VAD, Huh7 cells were treated with TRAIL (75ng/ml) for 24 h. (A-B) Western blots were used to determine the target proteins. (C) Western blot was used to detect the protein level of cFLIP_L. (D) Real time PCR was used to detect the mRNA level of cFLIP_L. Each value represents the mean ± SEM of three independent experiments performed in triplicate.*P<0.05 and **P<0.01.