Characterization of C-kit⁺ CSCs, MSCs, and exosomes.

<p>(a) Phase morphology of C-kit⁺ CSCs (Olympus, Japan); scale bar = 100 μm. (b) Representative flow cytometric characterization of C-kit⁺ CSCs for the typical surface antigens and isotype control after magnetic bead sorting. surface expression of C-kit, and absence of surface expression of CD45, CD34. (c) MSC morphology was observed under a microscope (Olympus, Japan); scale bar = 100 μm. (d) MSCs were characterized by flow cytometric analysis for typical surface antigens or isotype control: surface expression of CD29, CD90,and absence of surface expression of CD45. (e) A transmission electron microscope was used to analyze MSC-derived exosomes. Images show a round-shaped vesicle with a diameter of approximately 100 nm. Scale bar = 100 nm/50 nm. (f) Western blotting characterization of the CD63, CD9, and Hsp70 MSC-Exos markers.