Expression variations of genes involved in IGF/PI3K, FGFMAPK, TGF-β and Wnt signaling pathways in hESC lines H7, HUES1, HUES8 and HUES9.

<p>(A) Fold change of BMP, WNT, FDZ and FGF family genes among the four lines. * represents genes differentially expressed at least between two cell lines by FC > 2 and FDR < 0.01. (B) Percentage of DEGs in PUGs and PDGs of the four signaling pathways. Statistically significant were analyzed by student's t-test. (C) Line chart showing fold change of differentially expressed WNT family genes and inhibitor DKK1 in Wnt signaling pathway in the four cell lines. (D) Line chart depicting fold change of BMP4 and pluripotent factors POU5F1 and NANOG in the four cell lines. Fold change of each gene mentioned here was normalized by the minimum CPM of the gene among the four lines.