Astrocytes express a ligand for NKp44 and increasing concentrations of HIV 3S peptide decreases its expression.

<p>(A) The expression of a ligand for NKp44 (NKp44L) on astrocytes was established using 70 μg of NKp44-Ig fusion protein detected by anti-IgG-Fc-PE (filled histogram). Astrocytes incubated with 2.5 μg anti-IgG-Fc-PE were used as negative controls (open histogram). Cells were tested for the binding of NKp44-Ig fusion protein with no stimulation, 1 μg/ml peptide stimulation, and 10 μg/ml stimulation. Mean Fluorescent Intensity (MFI) values, the differences between the mean value for the control group and the experimental groups were quantified and MFI is indicated in the top right corner of all plots. Fig A is representative of 1 sample. (B) The combined MFIs of tests done in duplicate on 5 different astrocyte donors. Data are represented as means ± SEM, **** p < 0.0001, ANOVA.