Specific binding of M2pep to and internalization into <i>in vitro</i> differentiated M2 macrophages.

<p>(<b>A</b>) M1 and M2 macrophages were incubated with M2pep or scM2pep (20 μM) for 30 min and analyzed by flow cytometry. (M2pep: n = 6; scM2pep: n = 4). (<b>B</b>) Representative immunofluorescence images of M1 and M2 macrophages incubated with 200 μM M2pep for 30 min (blue–Hoechst 33342; red–M2pep; green–phalloidin; white–F4/80; Scale 10 μm). The percentage of M2pep positive macrophages was quantified by counting the PE-CF594 positive cells in the M1 or M2 macrophage population (M1/M2 n = 10). Values are means ± SEM; *P<0.05. Statistical analysis was performed with the Wilcoxon-matched pair test.