Rhodomyrtone acts directly on the lipid bilayer without specific head group preference.

<p><b>(A)</b> Minimal inhibitory concentrations (μg/ml) of rhodomyrtone against different lipid head group mutants of <i>B</i>. <i>subtilis</i>. Daptomycin was used as a control. <b>(B)</b> Laurdan GP spectroscopy of liposomes prepared from <i>E</i>. <i>coli</i> polar lipid extract exposed to different concentrations of rhodomyrtone. Compound to lipid ratios: 1 μg/ml = 1:400, 10 μg/ml = 1:40, 50 μg/ml = 1:7. <b>(C)</b> Laurdan spectroscopy of liposomes treated with the membrane fluidizer benzyl alcohol. Compound to lipid ratios: 10 mM = 12.5:1, 30 mM = 37.5:1, 50 mM = 62.5:1. <b>(D)</b> SIM microscopy images of liposomes prepared from <i>E</i>. <i>coli</i> polar lipid extract exposed to different concentrations of rhodomyrtone for 5 min. Liposomes were stained with DiIC12. Compound to lipid ratios as in <b>(B)</b>. Upper and lower rows show two examples from the same samples. Scale bar 1 μm. <b>(E)</b> Diameter of liposomes from <b>(D i)</b> and <b>(D ii)</b>. Black bars represent mean with SEM.