MOESM1 of Construction of an organelle-like nanodevice via supramolecular self-assembly for robust biocatalysts

Additional file 1: Table S1. Primers for cloning, mutagenesis of the MhIHL and for construction of the nanoreactor. Fig. S1. Schematic and sequence of artificially fused protein open reading frame for γ-lactamase nanoreactor. Fig. S2. Size-exclusion chromatography (SEC) of the free and encapsulated (+)-γ-lactamases. Fig. S3. TEM structures of free γ-lactamase and encapsulated γ-lactamase self-assembled in vitro. Fig. S4. AFM structures of free γ-lactamase and encapsulated γ-lactamase self-assembled in vitro. Fig. S5. Structures of empty protein dodecahedron formed by the engineered ketohydroxyglutarate aldolase from Thermotoga maritima. Fig. S6. Michaelis–Menten plot used to determine the Km and kcat values. Fig. S7. Optimal temperature for free γ-lactamase and encapsulated γ-lactamase. Fig. S8. Optimal pH for free γ-lactamase and encapsulated γ-lactamase. Fig. S9. Comparison of the stability of the free and encapsulated γ-lactamase in presence of protease. Fig. S10. The Lineweaver–Burk double reciprocal plot for different concentrations of (+)-γ-lactam