Expression of CSN gene products and CUL1 modification status in various viable <i>csn</i> mutants.

<p><b>(A)</b> Apical apex tissues from indicated mutants were extracted and the proteins examined by immunoblotting using indicated antibodies. Lower levels of the CSN (CSN3 and CSN5A) and hyper-neddylation of CUL1 were found in <i>csn5a-1</i>, <i>csn5a-2</i>, <i>csn1-10</i>, and <i>csn2-5</i>. <b>(B)</b> Light-grown seedlings (4-day) of indicated genotypes were used for immunoblotting with anti-CSN5B. The antibody strongly reacted with CSN5B, but also cross-reacted with CSN5A. Endogenous CSN5B migrated slower than CSN5A on the SDS-PAGE. See also <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007237#pgen.1007237.s004" target="_blank">S1 Fig</a>.