A decrease of <i>MHCII</i>, <i>Dl4</i>, and <i>Ccl25</i> but an increase in <i>Il7</i> in the <i>Foxn1</i>^<i>lacZ</i> thymus.

<p><b>(A).</b> The gate for CD45^-Epcam⁺MHCII⁺ TEC. <b>(B-E).</b> TECs sorted from day 8, 15, 22, 30 and 40 thymi were analyzed for gene expression of <i>MHCII (B)</i>, <i>Dl4 (C)</i>, <i>Ccl25(D)</i> and <i>Il7 (E)</i> respectively by qPCR. The gene expression in TECs at all time points were normalized to day 8 +/Z control values, which were set as 1. Each time point represents at least five individuals. <b>(F).</b> TECs were sorted into MHCII^lo and MHCII^hi populations from day 22 thymi. <i>Il7</i> gene expression in each sorted population is shown to the right. <b>(G).</b> TECs were sorted into four populations defined as MHCII^lo, MHCII^hi, cTECs (UEA-1^-), and mTECs (UEA-1⁺) from day 30 thymi. <i>Il7</i> expression levels in these sorted populations is shown in the histogram. (+/Z: n = 2, Z/Z: n = 3 in F and G). Student’s t-test results between +/Z control and Z/Z cells: *P <0.05, **P <0.01, ***P <0.001. Bars indicate means ± SEM.