Binding of EBNA2 to viral and host loci is influenced by EBNA-LP.

<p>ChIP analyses of EBNA2 at promoters regulated by EBNA2 in LPKO^w- and WT^w-infected cells. <b>A.</b> Schematic representation of the EBV genome region containing latency promoters, showing positions of qPCR assays used for the ChIP. Dashed regions of the schematic are shown shorter than in reality. <b>B.</b> ChIP for EBNA2 in adult B cells five days post infection with WT^w (orange) and LPKO^w (black). Single letters are EBV locations per Fig 8A. EBNA2 binding sites in the host IL7 and the enhancer (En) and promoter (Pr) regions of HES1 genes are shown, with myoglobin (MG) and MCM6 (M6) genes as negative controls. <b>C.</b> Time course after LPKO^w and WT^w infection for EBNA2 binding to IL7 as a representative host gene locus. <b>D.</b> Time courses for viral promoters for LMP2A (assay A), LMP1/2B (assay B) and the RBPJK-binding site in Cp (assay R). All error bars in B-D show ± 1 standard deviation from the mean. Complete data sets are in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006890#ppat.1006890.s021" target="_blank">S4 Data</a>.