Evaluation of C1q, fB, and MBL-A changes in kidneys.

<p>(A) Immunofluorescence staining for three complement activator-associated proteins. (B) C1q, fB, and MBL-A protein levels were measured using western blot analysis; the ratio of complement expression to that of GAPDH was determined using densitometry (n = 6). (C) The mRNA expression levels of these proteins in the different groups. Protein and RNA levels in the kidneys of AKI rats were higher than those in the control group, but the amplitude of this increase was lower after the depletion of complement. ★ P<0.01, ▲ P>0.05 vs. control; ◆ P<0.01 vs. AKI.