Intracellular production of ROS during disease development in pathosystems I-III.

<p>ROS production as visualized using H₂DCF-DA in control and infected potato sprouts, soybean hypocotyls and leaves along with mycelia of control <i>R</i>. <i>solani</i> AG-3, AG-4, AG1-IA, grown on half-strength PDA overlaid with cellophane membranes. (<b>A-B</b>) Control mycelia with no evidence of endogenous ROS at 120 h, (<b>G-H</b>) 36 h, and (<b>M-N</b>) 18 h upon growth on PDA membrane overlaid with cellophane membranes. <b>(C-D)</b> Control <i>S</i>. <i>tuberosum</i> sprout, (<b>I-J</b>) <i>G</i>. <i>max</i> hypocotyl and (<b>O-P</b>) <i>G</i>. <i>max</i> leaf with no evidence of endogenous ROS. Mycelia of <i>R</i>. <i>solani</i> during disease development in pathosystems I-III (<b>Fig E-F, Fig K-L</b>, <b>and Fig Q-R</b>, respectively). Bar = 500 μm.