MOESM3 of Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

Additional file 20 of Unraveling axonal mechanisms of traumatic brain injury

Victorio M. Pozo Devoto (13842200)
Valentina Lacovich (13842203)
Monica Feole (13842206)
Pratiksha Bhat (13842209)
Jaroslav Chovan (13842212)
Maria Čarna (13842215)
Isaac G. Onyango (13842218)
Neda Dragišić (13842221)
Martina Sűsserová (13842224)
Martin E. Barrios-Llerena (2540353)
Gorazd B. Stokin (9129836)

September 2022

Additional file 20. Key Resources Table: Table including antibodies, resources, kits, cells, and sof...

Additional file 3: of Differential neuro-immune patterns in two clinically relevant murine models of multiple sclerosis

Krista DiSano (6727508)
Michael Linzey (6727511)
Darlene Royce (372501)
Andrew Pachner (6727514)
Francesca Gilli (253853)

May 2019

Table S3. Subclusters of genes/probes showing the most differential expression patterns between TMEV...

Additional file 2: of A scalable solution for isolating human multipotent clinical-grade neural stem cells from ES precursors

Dasa Bohaciakova (6456569)
Marian Hruska-Plochan (142724)
Rachel Tsunemoto (6456575)
Wesley Gifford (3416579)
Shawn Driscoll (6456578)
Thomas Glenn (6456581)
Stephanie Wu (532998)
Silvia Marsala (142718)
Michael Navarro (5827589)
Takahiro Tadokoro (6456584)
Stefan Juhas (334409)
Jana Juhasova (334411)
Oleksandr Platoshyn (270714)
David Piper (6456587)
Vickie Sheckler (6456590)
Dara Ditsworth (6456593)
Samuel Pfaff (3416582)
Martin Marsala (142733)

March 2019

Table S2. Antibodies used for flow cytometry, FACS, and immunofluorescence staining. (PDF 26 kb

MOESM1 of Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

Justin Rustenhoven (3514034)
Leon Smyth (3551456)
Deidre Jansson (3551450)
Patrick Schweder (4877305)
Miranda Aalderink (452760)
Emma Scotter (3514046)
Edward Mee (296153)
Richard Faull (3514040)
Thomas Park (3514031)
Mike Dragunow (160417)

February 2018

Additional file 1: Table S1. In vitro culture conditions of brain pericytes in selected publications...

MOESM4 of Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

Justin Rustenhoven (3514034)
Leon Smyth (3551456)
Deidre Jansson (3551450)
Patrick Schweder (4877305)
Miranda Aalderink (452760)
Emma Scotter (3514046)
Edward Mee (296153)
Richard Faull (3514040)
Thomas Park (3514031)
Mike Dragunow (160417)

February 2018

Additional file 4: Table S4. List of primers used for qRT-PCR. This table lists the primers used for...

Additional file 1 of Assessment of technical and clinical utility of a bead-based flow cytometry platform for multiparametric phenotyping of CNS-derived extracellular vesicles

Alexandra Brahmer (17110139)
Carsten Geiß (16841153)
Andriani Lygeraki (16841994)
Elmo Neuberger (13895505)
Theophilos Tzaridis (17110142)
Tinh Thi Nguyen (11521768)
Felix Luessi (596770)
Anne Régnier-Vigouroux (16841156)
Gunther Hartmann (308665)
Perikles Simon (363393)
Kristina Endres (532488)
Stefan Bittner (376727)
Katrin S. Reiners (17110145)
Eva-Maria Krämer-Albers (76630)

October 2023

Additional file 1: Table S1. Capture bead composition of the MACSPlex EV Kit Neuro, human, panel A a...

MOESM1 of Modeling and rescue of defective bloodâbrain barrier function of induced brain microvascular endothelial cells from childhood cerebral adrenoleukodystrophy patients

Catherine Lee (247739)
Hannah Seo (5055659)
Anibal Armien (280792)
Frank Bates (2540284)
Jakub Tolar (298070)
Samira Azarin (5055662)

April 2018

Additional file 1: Table S1. Information on induced pluripotent stem cell (iPSC) lines used in study...

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

A. Cossarizza
H.-. Chang
A. Radbruch
S. Abrignani
R. Addo
M. Akdis
I. Andra
F. Andreata
F. Annunziato
E. Arranz
P. Bacher
S. Bari
V. Barnaba
J. Barros-Martins
D. Baumjohann
C.G. Beccaria
D. Bernardo
D.A. Boardman
J. Borger
C. Bottcher
L. Brockmann
M. Burns
D.H. Busch
G. Cameron
I. Cammarata
A. Cassotta
Y. Chang
F.G. Chirdo
E. Christakou
L. Cicin-Sain
L. Cook
A.J. Corbett
R. Cornelis
L. Cosmi
M.S. Davey
S. De Biasi
G. De Simone
G. del Zotto
M. Delacher
F. Di Rosa
J.D. Santo
A. Diefenbach
J. Dong
T. Dorner
R.J. Dress
C.-. Dutertre
S.B.G. Eckle
P. Eede
M. Evrard
C.S. Falk
M. Feuerer
S. Fillatreau
A. Fiz-Lopez
M. Follo
G.A. Foulds
J. Frobel
N. Gagliani
G. Galletti
A. Gangaev
N. Garbi
J.A. Garrote
J. Geginat
N.A. Gherardin
L. Gibellini
F. Ginhoux
D.I. Godfrey
P. Gruarin
C. Haftmann
L. Hansmann
C.M. Harpur
A.C. Hayday
G. Heine
D.C. Hernandez
M. Herrmann
O. Hoelsken
Q. Huang
S. Huber
J.E. Huber
J. Huehn
M. Hundemer
W.Y.K. Hwang
M. Iannacone
S.M. Ivison
H.-. Jack
P.K. Jani
B. Keller
N. Kessler
S. Ketelaars
L. Knop
J. Knopf
H.-. Koay
K. Kobow
K. Kriegsmann
H. Kristyanto
A. Krueger
J.F. Kuehne
H. Kunze-Schumacher
P. Kvistborg
I. Kwok
D. Latorre
D. Lenz
M.K. Levings
A.C. Lino
F. Liotta
H.M. Long
E. Lugli
K.N. MacDonald
L. Maggi
M.K. Maini
F. Mair
C. Manta
R.A. Manz
M.-. Mashreghi
A. Mazzoni
J. McCluskey
H.E. Mei
F. Melchers
S. Melzer
D. Mielenz
L. Monin
L. Moretta
G. Multhoff
L.E. Munoz
M. Munoz-Ruiz
F. Muscate
A. Natalini
K. Neumann
L.G. Ng
A. Niedobitek
J. Niemz
L.N. Almeida
S. Notarbartolo
L. Ostendorf
L.J. Pallett
A.A. Patel
G.I. Percin
G. Peruzzi
M. Pinti
A.G. Pockley
K. Pracht
I. Prinz
I. Pujol-Autonell
N. Pulvirenti
L. Quatrini
K.M. Quinn
H. Radbruch
H. Rhys
M.B. Rodrigo
C. Romagnani
C. Saggau
S. Sakaguchi
F. Sallusto
L. Sanderink
I. Sandrock
C. Schauer
A. Scheffold
H.U. Scherer
M. Schiemann
F.A. Schildberg
K. Schober
J. Schoen
W. Schuh
T. Schuler
A.R. Schulz
S. Schulz
J. Schulze
S. Simonetti
J. Singh
K.M. Sitnik
R. Stark
S. Starossom
C. Stehle
F. Szelinski
L. Tan
A. Tarnok
J. Tornack
T.I.M. Tree
J.J.P. van Beek
W. van de Veen
K. van Gisbergen
C. Vasco
N.A. Verheyden
A. von Borstel
K.A. Ward-Hartstonge
K. Warnatz
C. Waskow
A. Wiedemann
A. Wilharm
J. Wing
O. Wirz
J. Wittner
J.H.M. Yang
J. Yang

January 2021

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing ...

MOESM3 of Analysis of PD-1 related immune transcriptional profile in different cancer types

Jun Shang (1818454)
Qian Song (703098)
Zuyi Yang (6161654)
Xiaoyan Sun (28501)
Meijuan Xue (6161657)
Wenjie Chen (130753)
Jingcheng Yang (671953)
Sihua Wang (307155)

December 2018

Additional file 3: Fig. S3. The immune related GO terms analysis of each type of cancer

MOESM3 of Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice

Micely Hermida (4664479)
Rafaela Malta (4664476)
Marcos S. Santos (4664473)
Washington dos-Santos (688358)

December 2017

Additional file 3. Cell immunophenotyping: To identify the neutrophils expressed F4/80 antigens, R4 ...

Additional file 3: of A pilot study on the usefulness of peripheral blood flow cytometry for the diagnosis of lower risk myelodysplastic syndromes: the “MDS thermometer”

Ana Aires (4959586)
Maria dos Anjos Teixeira (4959577)
Catarina Lau (4959574)
Cláudia Moreira (281003)
Ana Spínola (4959580)
Alexandra Mota (4959583)
Inês Freitas (4824126)
Jorge Coutinho (813325)
Margarida Lima (409636)

March 2018

Table S3. FSC and SSC values and MedFI of expression of the molecules under study on monocytes from ...

Additional file 1 of Media matters: culture medium-dependent hypervariable phenotype of mesenchymal stromal cells

Joan C. Fitzgerald (12217589)
Georgina Shaw (716701)
J. Mary Murphy (17625388)
Frank Barry (13855320)

December 2023

Additional file 1. Supplementary Information 1: Antibody details for flow cytometric characterisatio...

MOESM1 of Deep sustained response to daratumumab monotherapy associated with T-cell expansion in triple refractory myeloma

Saad Usmani (4838196)
Imran Khan (109715)
Christopher Chiu (543836)
David Foureau (4838199)
Lawrence Druhan (4838205)
Katherine Rigby (4838208)
Tineke Casneuf (24442)
A. Sasser (4838202)

February 2018

Additional file 1: Table S1. Antibodies used for flow cytometryâbased minimal residual disease ass...

Additional file 1: of Blood-brain barrier hyperpermeability precedes demyelination in the cuprizone model

Stefan Berghoff (4637743)
Tim DĂźking (4637725)
Lena Spieth (4637746)
Jan Winchenbach (4637740)
Sina Stumpf (280304)
Nina Gerndt (4637728)
Kathrin Kusch (4637734)
Torben Ruhwedel (220465)
Wiebke MĂśbius (4637731)
Gesine Saher (4637737)

December 2017

Table S1. Primer sequences used for gene expression analyses. Table S2. Quantification of gene expre...

List of Antibodies used for flow cytometry and immunofluorescence analysis.

Marina Gabriela Monteiro Carvalho Mori da Cunha (785732)
Silvia Zia (785733)
Fanny Oliveira Arcolino (785734)
Marianne Sylvia Carlon (785735)
Diego Vilibaldo Beckmann (785736)
Ney Luis Pippi (785737)
Dominguita Luhers Graça (785738)
Elena Levtchenko (311960)
Jan Deprest (548204)
Jaan Toelen (311961)

August 2015

<p>List of Antibodies used for flow cytometry and immunofluorescence analysis.</p

Additional file 20 of Unraveling axonal mechanisms of traumatic brain injury

Victorio M. Pozo Devoto (13842200)
Valentina Lacovich (13842203)
Monica Feole (13842206)
Pratiksha Bhat (13842209)
Jaroslav Chovan (13842212)
Maria Čarna (13842215)
Isaac G. Onyango (13842218)
Neda Dragišić (13842221)
Martina Sűsserová (13842224)
Martin E. Barrios-Llerena (2540353)
Gorazd B. Stokin (9129836)

September 2022

Additional file 20. Key Resources Table: Table including antibodies, resources, kits, cells, and sof...

Additional file 3: of Differential neuro-immune patterns in two clinically relevant murine models of multiple sclerosis

Krista DiSano (6727508)
Michael Linzey (6727511)
Darlene Royce (372501)
Andrew Pachner (6727514)
Francesca Gilli (253853)

May 2019

Table S3. Subclusters of genes/probes showing the most differential expression patterns between TMEV...

Additional file 2: of A scalable solution for isolating human multipotent clinical-grade neural stem cells from ES precursors

Dasa Bohaciakova (6456569)
Marian Hruska-Plochan (142724)
Rachel Tsunemoto (6456575)
Wesley Gifford (3416579)
Shawn Driscoll (6456578)
Thomas Glenn (6456581)
Stephanie Wu (532998)
Silvia Marsala (142718)
Michael Navarro (5827589)
Takahiro Tadokoro (6456584)
Stefan Juhas (334409)
Jana Juhasova (334411)
Oleksandr Platoshyn (270714)
David Piper (6456587)
Vickie Sheckler (6456590)
Dara Ditsworth (6456593)
Samuel Pfaff (3416582)
Martin Marsala (142733)

March 2019

Table S2. Antibodies used for flow cytometry, FACS, and immunofluorescence staining. (PDF 26 kb

MOESM1 of Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

Justin Rustenhoven (3514034)
Leon Smyth (3551456)
Deidre Jansson (3551450)
Patrick Schweder (4877305)
Miranda Aalderink (452760)
Emma Scotter (3514046)
Edward Mee (296153)
Richard Faull (3514040)
Thomas Park (3514031)
Mike Dragunow (160417)

February 2018

Additional file 1: Table S1. In vitro culture conditions of brain pericytes in selected publications...

MOESM4 of Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

Justin Rustenhoven (3514034)
Leon Smyth (3551456)
Deidre Jansson (3551450)
Patrick Schweder (4877305)
Miranda Aalderink (452760)
Emma Scotter (3514046)
Edward Mee (296153)
Richard Faull (3514040)
Thomas Park (3514031)
Mike Dragunow (160417)

February 2018

Additional file 4: Table S4. List of primers used for qRT-PCR. This table lists the primers used for...

Additional file 1 of Assessment of technical and clinical utility of a bead-based flow cytometry platform for multiparametric phenotyping of CNS-derived extracellular vesicles

Alexandra Brahmer (17110139)
Carsten Geiß (16841153)
Andriani Lygeraki (16841994)
Elmo Neuberger (13895505)
Theophilos Tzaridis (17110142)
Tinh Thi Nguyen (11521768)
Felix Luessi (596770)
Anne Régnier-Vigouroux (16841156)
Gunther Hartmann (308665)
Perikles Simon (363393)
Kristina Endres (532488)
Stefan Bittner (376727)
Katrin S. Reiners (17110145)
Eva-Maria Krämer-Albers (76630)

October 2023

Additional file 1: Table S1. Capture bead composition of the MACSPlex EV Kit Neuro, human, panel A a...

MOESM1 of Modeling and rescue of defective bloodâbrain barrier function of induced brain microvascular endothelial cells from childhood cerebral adrenoleukodystrophy patients

Catherine Lee (247739)
Hannah Seo (5055659)
Anibal Armien (280792)
Frank Bates (2540284)
Jakub Tolar (298070)
Samira Azarin (5055662)

April 2018

Additional file 1: Table S1. Information on induced pluripotent stem cell (iPSC) lines used in study...

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

A. Cossarizza
H.-. Chang
A. Radbruch
S. Abrignani
R. Addo
M. Akdis
I. Andra
F. Andreata
F. Annunziato
E. Arranz
P. Bacher
S. Bari
V. Barnaba
J. Barros-Martins
D. Baumjohann
C.G. Beccaria
D. Bernardo
D.A. Boardman
J. Borger
C. Bottcher
L. Brockmann
M. Burns
D.H. Busch
G. Cameron
I. Cammarata
A. Cassotta
Y. Chang
F.G. Chirdo
E. Christakou
L. Cicin-Sain
L. Cook
A.J. Corbett
R. Cornelis
L. Cosmi
M.S. Davey
S. De Biasi
G. De Simone
G. del Zotto
M. Delacher
F. Di Rosa
J.D. Santo
A. Diefenbach
J. Dong
T. Dorner
R.J. Dress
C.-. Dutertre
S.B.G. Eckle
P. Eede
M. Evrard
C.S. Falk
M. Feuerer
S. Fillatreau
A. Fiz-Lopez
M. Follo
G.A. Foulds
J. Frobel
N. Gagliani
G. Galletti
A. Gangaev
N. Garbi
J.A. Garrote
J. Geginat
N.A. Gherardin
L. Gibellini
F. Ginhoux
D.I. Godfrey
P. Gruarin
C. Haftmann
L. Hansmann
C.M. Harpur
A.C. Hayday
G. Heine
D.C. Hernandez
M. Herrmann
O. Hoelsken
Q. Huang
S. Huber
J.E. Huber
J. Huehn
M. Hundemer
W.Y.K. Hwang
M. Iannacone
S.M. Ivison
H.-. Jack
P.K. Jani
B. Keller
N. Kessler
S. Ketelaars
L. Knop
J. Knopf
H.-. Koay
K. Kobow
K. Kriegsmann
H. Kristyanto
A. Krueger
J.F. Kuehne
H. Kunze-Schumacher
P. Kvistborg
I. Kwok
D. Latorre
D. Lenz
M.K. Levings
A.C. Lino
F. Liotta
H.M. Long
E. Lugli
K.N. MacDonald
L. Maggi
M.K. Maini
F. Mair
C. Manta
R.A. Manz
M.-. Mashreghi
A. Mazzoni
J. McCluskey
H.E. Mei
F. Melchers
S. Melzer
D. Mielenz
L. Monin
L. Moretta
G. Multhoff
L.E. Munoz
M. Munoz-Ruiz
F. Muscate
A. Natalini
K. Neumann
L.G. Ng
A. Niedobitek
J. Niemz
L.N. Almeida
S. Notarbartolo
L. Ostendorf
L.J. Pallett
A.A. Patel
G.I. Percin
G. Peruzzi
M. Pinti
A.G. Pockley
K. Pracht
I. Prinz
I. Pujol-Autonell
N. Pulvirenti
L. Quatrini
K.M. Quinn
H. Radbruch
H. Rhys
M.B. Rodrigo
C. Romagnani
C. Saggau
S. Sakaguchi
F. Sallusto
L. Sanderink
I. Sandrock
C. Schauer
A. Scheffold
H.U. Scherer
M. Schiemann
F.A. Schildberg
K. Schober
J. Schoen
W. Schuh
T. Schuler
A.R. Schulz
S. Schulz
J. Schulze
S. Simonetti
J. Singh
K.M. Sitnik
R. Stark
S. Starossom
C. Stehle
F. Szelinski
L. Tan
A. Tarnok
J. Tornack
T.I.M. Tree
J.J.P. van Beek
W. van de Veen
K. van Gisbergen
C. Vasco
N.A. Verheyden
A. von Borstel
K.A. Ward-Hartstonge
K. Warnatz
C. Waskow
A. Wiedemann
A. Wilharm
J. Wing
O. Wirz
J. Wittner
J.H.M. Yang
J. Yang

January 2021

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing ...

MOESM3 of Analysis of PD-1 related immune transcriptional profile in different cancer types

Jun Shang (1818454)
Qian Song (703098)
Zuyi Yang (6161654)
Xiaoyan Sun (28501)
Meijuan Xue (6161657)
Wenjie Chen (130753)
Jingcheng Yang (671953)
Sihua Wang (307155)

December 2018

Additional file 3: Fig. S3. The immune related GO terms analysis of each type of cancer

MOESM3 of Selecting the right gate to identify relevant cells for your assay: a study of thioglycollate-elicited peritoneal exudate cells in mice

Micely Hermida (4664479)
Rafaela Malta (4664476)
Marcos S. Santos (4664473)
Washington dos-Santos (688358)

December 2017

Additional file 3. Cell immunophenotyping: To identify the neutrophils expressed F4/80 antigens, R4 ...

Additional file 3: of A pilot study on the usefulness of peripheral blood flow cytometry for the diagnosis of lower risk myelodysplastic syndromes: the “MDS thermometer”

Ana Aires (4959586)
Maria dos Anjos Teixeira (4959577)
Catarina Lau (4959574)
Cláudia Moreira (281003)
Ana Spínola (4959580)
Alexandra Mota (4959583)
Inês Freitas (4824126)
Jorge Coutinho (813325)
Margarida Lima (409636)

March 2018

Table S3. FSC and SSC values and MedFI of expression of the molecules under study on monocytes from ...

Additional file 1 of Media matters: culture medium-dependent hypervariable phenotype of mesenchymal stromal cells

Joan C. Fitzgerald (12217589)
Georgina Shaw (716701)
J. Mary Murphy (17625388)
Frank Barry (13855320)

December 2023

Additional file 1. Supplementary Information 1: Antibody details for flow cytometric characterisatio...

MOESM1 of Deep sustained response to daratumumab monotherapy associated with T-cell expansion in triple refractory myeloma

Saad Usmani (4838196)
Imran Khan (109715)
Christopher Chiu (543836)
David Foureau (4838199)
Lawrence Druhan (4838205)
Katherine Rigby (4838208)
Tineke Casneuf (24442)
A. Sasser (4838202)

February 2018

Additional file 1: Table S1. Antibodies used for flow cytometryâbased minimal residual disease ass...

Additional file 1: of Blood-brain barrier hyperpermeability precedes demyelination in the cuprizone model

Stefan Berghoff (4637743)
Tim DĂźking (4637725)
Lena Spieth (4637746)
Jan Winchenbach (4637740)
Sina Stumpf (280304)
Nina Gerndt (4637728)
Kathrin Kusch (4637734)
Torben Ruhwedel (220465)
Wiebke MĂśbius (4637731)
Gesine Saher (4637737)

December 2017

Table S1. Primer sequences used for gene expression analyses. Table S2. Quantification of gene expre...

List of Antibodies used for flow cytometry and immunofluorescence analysis.

Marina Gabriela Monteiro Carvalho Mori da Cunha (785732)
Silvia Zia (785733)
Fanny Oliveira Arcolino (785734)
Marianne Sylvia Carlon (785735)
Diego Vilibaldo Beckmann (785736)
Ney Luis Pippi (785737)
Dominguita Luhers Graça (785738)
Elena Levtchenko (311960)
Jan Deprest (548204)
Jaan Toelen (311961)

August 2015

<p>List of Antibodies used for flow cytometry and immunofluorescence analysis.</p

Additional file 20 of Unraveling axonal mechanisms of traumatic brain injury

Victorio M. Pozo Devoto (13842200)
Valentina Lacovich (13842203)
Monica Feole (13842206)
Pratiksha Bhat (13842209)
Jaroslav Chovan (13842212)
Maria Čarna (13842215)
Isaac G. Onyango (13842218)
Neda Dragišić (13842221)
Martina Sűsserová (13842224)
Martin E. Barrios-Llerena (2540353)
Gorazd B. Stokin (9129836)

September 2022

Additional file 20. Key Resources Table: Table including antibodies, resources, kits, cells, and sof...

Additional file 3: of Differential neuro-immune patterns in two clinically relevant murine models of multiple sclerosis

Krista DiSano (6727508)
Michael Linzey (6727511)
Darlene Royce (372501)
Andrew Pachner (6727514)
Francesca Gilli (253853)

May 2019

Table S3. Subclusters of genes/probes showing the most differential expression patterns between TMEV...

Additional file 2: of A scalable solution for isolating human multipotent clinical-grade neural stem cells from ES precursors

Dasa Bohaciakova (6456569)
Marian Hruska-Plochan (142724)
Rachel Tsunemoto (6456575)
Wesley Gifford (3416579)
Shawn Driscoll (6456578)
Thomas Glenn (6456581)
Stephanie Wu (532998)
Silvia Marsala (142718)
Michael Navarro (5827589)
Takahiro Tadokoro (6456584)
Stefan Juhas (334409)
Jana Juhasova (334411)
Oleksandr Platoshyn (270714)
David Piper (6456587)
Vickie Sheckler (6456590)
Dara Ditsworth (6456593)
Samuel Pfaff (3416582)
Martin Marsala (142733)

March 2019

Table S2. Antibodies used for flow cytometry, FACS, and immunofluorescence staining. (PDF 26 kb

MOESM3 of Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

Abstract

Extracted data

MOESM3 of Modelling physiological and pathological conditions to study pericyte biology in brain function and dysfunction

Abstract

Extracted data

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