Preparation and physical characterization of GM1-coated nanoparticles.

<p>(A) Schematic of GM1-NP fabrication. Poly(lactic-co-glycolic acid) (PLGA) dissolved in acetonitrile (CH₃CN) is added to an aqueous solution containing GM1. After acetonitrile evaporation, nanoparticles with a polymeric core and a lipid shell are formed. (B) Intensity-weighted size distribution of representative preparations of GM1-NPs and control PEG-NPs, and PLGA-NPs with a PLGA core but without a lipid shell. (C) Zeta potential of the indicated nanoparticle preparations (n = 3; mean ± SD; *p<0.05 vs. PLGA-NPs). (D) Nanoparticle size measurements over two weeks of incubation in distilled water or PBS (n = 3; mean ± SD). (E) Transmission electron micrograph of GM1-NPs.