MRI and calculation of ADC and T2 values.

<p>a: Transverse T2-weighted MR image (TR/TE = 4000msec/77msec) of rat gastrocnemius muscle, outlined in ovals, when animal is in a prone position. b-d: A line (red arrows in (b)) is set across gastrocnemius muscle, outlined in ovals. A line scan diffusion spectrum was obtained to calculate the ADC value, in which the b-value is stepped from 0 to 2000 mm²/seconds in 32 steps (c). That approach was also used to calculate T2, in which TE was stepped from 16.6 to 82.6 msec in 32 steps (d). The area inside rectangles represents signals from gastrocnemius muscle. e,f: Signal intensities obtained from line-scan diffusion MRS data are plotted as an exponential curve. ADC and T2 values are calculated from the signal decay curve.