TLR4 activity of <i>B</i>. <i>anthracis</i> and <i>S</i>. <i>aureus</i> PGN.

<p><i>B</i>. <i>anthracis</i> (open bars) and <i>S</i>. <i>aureus</i> (closed bars) PGN samples were taken after the major steps of the purification process, washed three times with endotoxin free water, dried, weighed, resuspended in water and tested for TLR4 activity at a concentration of 10 μg/ml, using the HEK-TLR4 cell assay. Two separate PGN preparations for each species were tested. The data are expressed as the mean ± SEM fold difference TLR4 activity over the negative control, endotoxin free water. Statistical significance was determined by ANOVA with Bonferroni post test. All PGN samples were not significantly different in TLR4 activity than 10 pg/ml LPS.