Effect of hydrogen peroxide on cellular viability in PhS-treated cells.

<p>Cells were treated with indicated concentrations (0, 0.2, 0.5, 2, 5, 10 mmol/L) of PhS for 24 hours and then treated with hydrogen peroxide (0, 10, 20 μmol/L) for 5 hours. After treatment, cellular viability was evaluated using Cell Counting Kit-8. Data are expressed as percent of control. Data are shown as mean ± S.D. (n = 3). Data are representative of two independent experiments. Cont, control; PhS, phenyl sulfate; H2O2, hydrogen peroxide. Asterisks indicate significant differences compared to corresponding controls (i.e. each black, grey, or hatched column was compared with its respective black, grey, or hatched control column) (*p < 0.05, **p < 0.01).