Dose-dependent effect of buthionine sulfoximine on total glutathione level.

<p>Cells were treated with indicated concentrations (0, 0.001, 0.005, 0.02, 0.1 mmol/L) of buthionine sulfoximine or 10 mmol/L PhS for 24 hours. Cells were lysed and glutathione levels were measured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0193342#pone.0193342.g001" target="_blank">Fig 1</a>. Data are shown as mean ± S.D. (n = 3). Data are representative of two independent experiments. Cont, control; BSO, buthionine sulfoximine; PhS, phenyl sulfate. Asterisks indicate significant differences compared to control (**p < 0.01).