Additional file 2: of ISL1 overexpression enhances the survival of transplanted human mesenchymal stem cells in a murine myocardial infarction model

Figure S1. Transplantation of ISL1-hMSCs improved cardiac function (LVEDV, LVESV and CO) in amyocardial infarction (MI) model. *p < 0.05 vs. sham; #p < 0.05 vs. MI + PBS; & p < 0.05 vs. MI + ISL1-hMSCs. Figure S2. ISL1 overexpression reduced TUNEL-positive cardiomyocytes in infarct hearts. Scale bar = 50 μm. Figure S3. ISL1 overexpression reduced CD3+ T lymphocytes in infarct hearts. Scale bar = 50 μm. Figure S4. ISL1 overexpression reduced the number of CD68+ T lymphocytes in infarct hearts. Scale bar = 50 μm. Figure S5. ISL1 overexpression reduced inflammation cytokines TNFα, IL-6, and IL-10. Scale bar = 50 μm. Figure S6. ISL1 overexpression downregulated the proliferation and proinflammatory cytokine production of CD3+ T cells in vitro. *p < 0.05 vs. control; #p < 0.05 vs. Ctrl-hMSCs. Figure S7. Representative images and quantification of Bax, Bcl-2, cleaved caspase 3, and full-length caspase 3 in ISL1-hMSCs and Ctrl-hMSCs with or without H2O2. *p < 0.05 vs. H2O2 + Ctrl-hMSCs. Figure S8. Top 10 GO functions of upregulated (a) and downregulated (b) genes in ISL1-MSCs. Figure S9. Heat map display of secreted proteins with RPKM values of more than 100 in ISL1-hMSCs and Ctrl-hMSCs. Figure S10. The IGFBP3 inhibition assay showed a reduction in active IGFBP3 in ISL1-hMSCs-CM. *p < 0.05 vs. control; #p < 0.05 vs. H2O2; &p < 0.05 vs. H2O2 + ISL1-hMSCs. Scale bar = 100 μm. Figure S11. The anti-apoptotic effect of IGFBP3 in ISL1-hMSCs-CM on the human cardiomyocyte cell line AC16 subjected to oxidative injury. Apoptosis rate = (TUNEL positive nuclei / DAPI + nuclei) × 100%. *p < 0.05 vs. control; #p < 0.05 vs. H2O2; &p < 0.05 vs. H2O2 + Ctrl-hMSCs; @p < 0.05 vs. H2O2 + ISL1-hMSCs. Scale bar = 100 μm. DAPI: 4′,6- diamidino-2-phenylindole. (PPT 15681 kb