Dectin-1, MR and Dectin-2 mainly bind to the surface of <i>F</i>. <i>pedrosoi-</i>hyphae, but not the transformed sclerotic cells.

<p>(A-C) The binding of murine-derived Dectin-1, Mannose Receptor (MR), and Dectin-2 to <i>F</i>. <i>pedrosoi</i> cultured in ATCC 830 medium with 10⁻⁶ M PAF at 35°C for 30 days was detected respectively by PE-conjugated anti-murine Dectin-1 mAb (A), FITC-conjugated anti-murine MR mAb (B), and PE-conjugated anti-murine Dectin-2 mAb (C) using confocal microscope. Left column: fluorescence field; Middle column: bright field; Right column: merged images. Scale bar = 20 μm. The transformed sclerotic cells with cross-septation and swelling chlamydospores were indicated by red circle (A) or red arrows (B and C). The fungal cells incubated only with PE-conjugated anti-murine Dectin-1 or Dectin-2 mAb, or only with FITC-conjugated anti-murine MR mAb were set as blank control.