Flow cytometry analysis of surface PD1 expression on synovial tissue infiltrating immune cells.
- Yanxia Guo (1600771)
- Alice M. Walsh (4407958)
- Mary Canavan (4898695)
- Mihir D. Wechalekar (4407961)
- Suzanne Cole (217727)
- Xuefeng Yin (2102104)
- Brittney Scott (4898686)
- Mathew Loza (4898692)
- Carl Orr (4898683)
- Trudy McGarry (4898680)
- Michele Bombardieri (56532)
- Frances Humby (84694)
- Susanna M. Proudman (4407964)
- Costantino Pitzalis (23531)
- Malcolm D. Smith (4407955)
- Joshua R. Friedman (459461)
- Ian Anderson (151648)
- Loui Madakamutil (4898689)
- Douglas J. Veale (339506)
- Ursula Fearon (8519)
- Sunil Nagpal (1828357)
Publication date
February 2018
DOIAbstract
<p>FACS plot of PD1 expression on gated live CD45<sup>+</sup> lymphocytes (A), CD4<sup>+</sup> (B) and CD8<sup>+</sup> (C) is shown. Each FACS plot represents one individual patient. Data shown is from 4 independent patients, and pre-gated on live lymphocytes. The PD-1 fluorescence minus one (FMO) staining control for patient 4 has been shown for the CD45, CD4 and CD8 panels (A-C). D. Quantification of CD45<sup>+</sup>, CD4<sup>+</sup> and CD8<sup>+</sup> synovial infiltrating immune cells expressing PD-1 on surface is presented. Data is shown as a mean percentage of cells from 4 patients.</p
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